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| Effect of Eliminating Cancer Decoction and compatibility of his dismantle prescription on growth or apoptosis of MCF-7 breast cancer cells |
| Hits 1714 Download times 1943 Received:May 20, 2010 |
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| DOI
10.11656/j.issn.1672-1519.2010.05.24 |
| Key Words
benefiting vital QI and anti-cancer;detoxication and removing stasis;chemotherapy;Eliminating Cancer Decoction |
| Author Name | Affiliation | | CHEN Jun | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | ZHANG Yun-chao | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | LI Xiao-jiang | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | 贾英杰 | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China |
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| Abstract
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| [Objective] To investigate the growth and apoptosis of MCF-7 breast cancer cell treated by Xiaoyantang(Eliminating Cancer Decoction) and every group of dismantle prescription and the effect combined with 5-fluorouracil.[Methods] Based on the effect of the Eliminate Cancer Decoction it was the divided into five groups: clearing away heat and detoxication group,activating blood circulation and removing stasis group,detoxicationa removing stasis group(clearing heat combined with activating blood circulation and removing stasis),benefiting vital QI and strengthening the body resistance group and whole prescription group.To investigate the growth and apop-tosis of MCF-7 breast cancer the MTT was used and for detecting the apoptosis by eliminating cancer decoction combined with 5-fluo-rouracil Hoechst 33258 method was used.[Result] All the medicine of each group had effect to inhibit the cancer cell proliferation and to promote apoptosis in different degree.The effect of clearing away heat and detoxication group was superior to the whole decoction group.After that they were detoxication and removing stasis group,activating blood circulationa and removing stasis group,benefiting vital QI and strengthening body resistance.When using with 5-fluorouracil synergetic or additive effect could be found.[Conclusion] It is demon-strated in vitro experiment that the cancer eliminating decoction can suppress the MCF-7 breast cancer growth and promote the apoptosis.Using with 5-Fu chemotherapy together it has effect-increasing action.The inhibitory effect on growth or effect-increasing action of Clear-ing away heat and detoxication group was similar to the whole prescription group,suggesting that the medicine of clearing away heat and detoxication was the effective component of the whole prescription for anti-cancer and chemotherapy strengthening effect. |
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