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| In vitro study on effects of astrocytes following treatment with ginkgolide B on chemotaxis of neural stem cells |
| Hits 1622 Download times 1815 Received:June 20, 2010 |
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| DOI
10.11656/j.issn.1672-1519.2010.05.31 |
| Key Words
ginkgolide B;neural stem cells;astrocytes;VEGF;chemotaxis |
| Author Name | Affiliation | | XU Qian | Tianjin University of TCM, Tianjin 300193, China | | ZHANG YAN-jun | 天津中医药大学中药学院, 天津, 300193 | | LIU Yang | 天津中医药大学中药学院, 天津, 300193 | | 庄朋伟 | 天津中医药大学中药学院, 天津, 300193 |
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| Abstract
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| [Objective] To study the effect of ginkgolide B(GB) on chemotaxis of neurall stem cells(NSCs) Through acting on the astrocyte in brain microcirculation and its mechanism.[Methods] Using a millicell Chamber the chemotactic migration was observed.Oxygen-glu-cose deprivation(GOD) was used for making the astrocyte cell injured.10,20 μM of GB were treated on AC for 12 hrs and then the num-ber migrated on the lower layer of Millicell chmber was counted.The expression of chemotaxis gene VEGF mRNA of AC was detected by reverse transcription polymerase chain reaction(RT-PCR).[Results] The migrated number of the GFP-NSC after acting on AC with GB(10,20 μM)were significantly increased(P<0.05).And the results of RT-PCR showed that the VEGF mRNA expression were up-regulated significantly by GB(10,20 μM).[Concluision] GB can promote the NSCs chemokinesis and increase migrating to the injuried AC.This process may be introduced by VEGF. |
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