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| Effects of salvianolic acid B on expression of tissue factor and plasminogen activator inhibitor-1 in ApoE-gene knock-out mice treated with STZ and high fat diet |
| Hits 2133 Download times 1923 Received:July 07, 2010 |
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| DOI
10.11656/j.issn.1672-1519.2010.05.32 |
| Key Words
salvianolic acid B;apolipoprotein E-gene knock-out mice;tissue factor plasminogen activator inhibitor-1 |
| Author Name | Affiliation | | YUAN Jun-li | The Hangzhou Road Community Health Service Center, Tianjin 300451, China | | GUO Mao-juan | 天津中医药大学, 天津, 300193 | | ZHENG Fang | 天津中医药大学, 天津, 300193 | | 顾立彦 | 天津中医药大学, 天津, 300193 | | 范英昌 | 天津中医药大学, 天津, 300193 |
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| Abstract
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| [Objective] To observe the effect of Salvianolic acid B on thrombogenesis of atheromatous plaque in mice with diabetic atherosclerosis and the possible mechanism.[Methods] Fifty two-month female ApoE-gene knock-out mice were intraperitoneally inject-ed with 200 mg/kg STZ and fed with high fat diet for 12 months.Then blood sugar level was measured and forty diabetic atherosclerosis mice were selected for the following treatment experiment.Diabetic atherosclerosis mice were randomly divided into four groups and the mice in each group were fed with same high fat diet and administrated per oral with different drugs for 8 weeks,named MOD group(dis-tilled water),SalBH [SalB 160 mg/(kg·d)] group,SalBM group[SalB 80 mg/(kg·d)] and Lovastatin[LVT 2.3 mg/(kg·d)] group.Then fast blood sugar level(FBG) was measured by using full automatic biochemistry instrument;the concentration of blood plasminogen activator inhibitor-1(PAI-1)was determined by ELISA techniques.Mice aortas were subjected to fixation and paraffin imbedding,then paraffin sections were made.The expression of tissue factor(TF)was measured by immunohistochemistry staining and then analyzed by Image-Pro Plus(IPP) version 6.0 system.[Results] Compared with the MOD group,average optical density(OD) value of TF reduced significantly in SalBH,SalBM and LVT treatment group(P<0.05);PAI-1 concentration reduced significantly in SalBM group(P<0.05).[Conclusions] Salvianolic Acid B can decrease the expression of TF in atheromatous plaque and concentration of blood PAI-1.The result suggested that Salvianolic Acid B may reduce thrombogenesis by above mechanism. |
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