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| Comparison of inhibitory effect of alcohol and water extracts in twelve kinds of Chinese herbal medicine on tyrosinase |
| Hits 1917 Download times 3955 Received:January 20, 2010 |
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| DOI
10.11656/j.issn.1672-1519.2010.05.33 |
| Key Words
cosmetic whitening traditional Chinese medicine;tyrosinase;L-Dopa |
| Author Name | Affiliation | | CHEN Xue-yan | Tianjin University of TCM, Tianjin 300193, China | | WANG Yan | Tianjin University of TCM, Tianjin 300193, China | | LI Jing | The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China | | 毕月玲 | Tianjin University of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] To compare the inhibitory activity on tyrosinase in vitro between 70% alcohol and water extracts of twelve kinds of Chinese herbal medicine.[Methods] Using the reaction of the test compounds in inhibiting tyrosinase and its catalytic substrate(L-Dopa) and so reducing melanin and taking vitamin C as a standard control the absorbed density of the reaction solutions at 475 nm were mea-sured.The inhibitory effect of each test substance on tyrosinase activity was determined through comparison of the absorbed density.[Results] All the twelve kinds of traditional Chinese medicines in vitro showed different inhibitory effect on tyrosinase,among them,the 70% alcohol extract of Glycyrrhiza uralensis,Ligusticum chuanxiong Hort,Rhizoma Alismatis,Rhizoma Atractylodis had better inhibitory effect on tyrosinase than the water extracted one.However,the water extract of these Chinese herbs,including Folium Mori Macrocepha-lae,Potia Alba,Flos Carthami,Radix Ampelopsis,Bombyx Batryticatus,Rheum and Radix AngelicaeSinensis had more inhibitory effect on tyrosinase than their alcohol extract.[Conclusion] Some Chinese herbs,having using value for make-up and skin-whitening was screened out. |
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