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| The effect of high glucose and fat on apoptosis of endothelial cell in umbilical vein and mitochondria membrane potential |
| Hits 1535 Download times 1787 Received:October 11, 2010 |
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| DOI
10.11656/j.issn.1672-1519.2010.06.29 |
| Key Words
AGEs;ox-LDL;apoptosis;mitochondria membrane potential |
| Author Name | Affiliation | | BAO Li-ying | Tianjin University of TCM, Tianjin 300193, China | | JIANG Xi-juan | Tianjin University of TCM, Tianjin 300193, China | | ZHENG Fang | Tianjin University of TCM, Tianjin 300193, China | | 范英昌 | Tianjin University of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] To study the mechanism of apoptosis of human umbilical vein endothelial cell(HUVEC) induced by AGEs and ox-LDL.[Methods] HUVECs were incubated at some concentration of AGEs and ox-LDL for 24 h.The apoptosis was observed by Hochest 33258 staining.The mitochondria membrane potential(MMP) was analyzed by flowcytometry after Rhodamine-123.[Results] AGEs and ox-LDL could increase apoptosis significantly and decrease mitochondria membrane potential of the cultured HUVECs significantly also(P<0.05).[Conclusion] The results indicated that AGEs and ox-LDL could promote endothelial cell apoptosis,the mechanism in which may be related to decreasing of mitochondria membrane potential. |
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