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| Extraction,isolation and purification of curcumins from curcuma longa |
| Hits 1642 Download times 3008 Received:July 15, 2010 |
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| DOI
10.11656/j.issn.1672-1519.2010.06.32 |
| Key Words
curumin;demethoxycurcumin;bisdemethoxycurcumin;separation;purification |
| Author Name | Affiliation | | LI Li | Tianjin University of TCM, Tianjin 300193, China | | GUO Da-li | Tianjin University of TCM, Tianjin 300193, China | | ZHU Xiao-wei | 天津中医药大学, 天津, 300193 | | 朱彩芳 | Tianjin University of TCM, Tianjin 300193, China | | 何灵通 | Tianjin University of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] To study the method of extraction,isolation and purification for curcumins from Curcum longa.[Methods] The total curcumins were extracted by ethanol after washing by water and petroleum ether.The extract was defatted.The crude bisdemethoxycurcumin and the mixture of curcumin and demethoxycurcumin were separated by dry silica gel column chromatography.Then bisdemethoxycurcumin was purified by wet silica gel column chromatography.Using TLC and acetone-water recrystallization,separation of curcumin and demethoxycurcumin were complished.[Results] Melting points of curumin,demethoxycurcumin and bisdemethoxycurcumin were 182～184℃,168～170℃ and 226～228℃ respectively.They all showed high content above 96% in HPLC chromatograph during three times of retention time.[Conclusion] The method of extraction and purification for curumin,demethoxycurcumin and bisdemethoxycurcumin was simple and efficient. |
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