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| Studies on culture of adventitious roots of liquorice |
| Hits 1818 Download times 2204 Received:September 20, 2010 |
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| DOI
10.11656/j.issn.1672-1519.2011.01.32 |
| Key Words
Glycyrrhiza Uralensis Fisch.G.Glabra L.;different explants;exogenous hormones;adventitious root |
| Author Name | Affiliation | | ZHANG Jian | School of Traditional Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Key laboratory of Industrial Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China | | GAO Wen-yuan | School of Traditional Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Key laboratory of Industrial Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China
College of Bioengineering, Tianjin University of Science and Technology, Tianjin 300457, China | | WANG Juan | School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China | | 吕连媛 | Key laboratory of Industrial Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China
College of Bioengineering, Tianjin University of Science and Technology, Tianjin 300457, China |
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| Abstract
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| [Objective] To study the ability of inducing adventitious roots of different explants and exogenous hormones in order to provide the fundamental researches for the culture of adventitious roots of liquorice.[Method] Cut the roots,leaves and stems with buds of sterile seeds and inoculate them to the induction medium with different exogenous hormones.Inspect the rooting abilities of different explants and try to propagate the roots on this basis.[Result] Different explants and different types and concentrations of exogenous auxins have different inducing ability to adventitious roots.Roots and leaves’ effects of inducing adventitious roots are better than that of stem with bud.NAA is better in favor of inducing adventitious roots than IBA.However,BA restraints the inducement of adventitious roots.Different types of explants have different fitting concentrations of auxin(NAA) in inducing roots.The preferable inducing effect is the concentration of NAA at 0.2~1 mg/L to root,3~5 mg/L to leaf,1~3 mg/L to stem with bud.[Conclusion] All different explants of liquorice can induce adventitious roots in the culture medium with exogenous auxin,however,exogenous cytokinin restraints the inducement of roots.The roots and leaves are ideal explants of liquorice for inducing adventitious roots. |
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