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Influence of acupuncture on [Ca2+]i in hippocampus neuron of rats with focal cerebral ischemia |
Hits 1827 Download times 1552 Received:January 29, 2012 |
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DOI
10.11656/j.issn.1672-1519.2012.02.13 |
Key Words
“XNKQ” acupuncture method;focal cerebral ischemia;hippocampus CA1 neuron;[Ca2+]i;calcium overloading;confocal |
Author Name | Affiliation | YANG Sha | Acupuncture Institute of the First Hospital Affiliated of Tianjin University of TCM, Acupuncture Encephalopathy Key Research Laboratory of the State Administration of TCM, Tianjin Acupuncture Key Laboratory, Tianjin 300193, China | SHEN Yan | Acupuncture Institute of the First Hospital Affiliated of Tianjin University of TCM, Acupuncture Encephalopathy Key Research Laboratory of the State Administration of TCM, Tianjin Acupuncture Key Laboratory, Tianjin 300193, China | SUN Yun-hao | Acupuncture Institute of the First Hospital Affiliated of Tianjin University of TCM, Acupuncture Encephalopathy Key Research Laboratory of the State Administration of TCM, Tianjin Acupuncture Key Laboratory, Tianjin 300193, China | WANG Shu | Acupuncture Institute of the First Hospital Affiliated of Tianjin University of TCM, Acupuncture Encephalopathy Key Research Laboratory of the State Administration of TCM, Tianjin Acupuncture Key Laboratory, Tianjin 300193, China |
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Abstract
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[Objective] To study the dynamic changes of the [Ca2+]i in hippocampus neurons of rats with focal cerebral ischemia and the effect of "XNKQ" acupuncture to explore the the rapeutic action of early intervention with "XNKQ" acupuncture.[Methods] MCAO was established in rats.They were treated with "XNKQ" acupuncture method.At different time point the dynamic changes of the [Ca2+]i in hippocampus CA1 neurons were examined with confocal laser scanning microscope.[Results] Compared with the control group,the two neuron [Ca2+]i of hippocampus CA1 regions of model group began to increase after ischemia for 1h,and sustained to elevate until 24 h(P<0.05),while the sham operated group had no difference(P>0.05).At the same time point,there was no difference of [Ca2+]i between non-acupoint group and model group(P>0.05),while the "XNKQ" acupuncture group was significantly lower than that of the model group(P<0.05,P<0.01).[Conclusion] The [Ca2+]i in hippocampus CA1 neuron of rats is increased persistently during acute focal cerebralis chemia,causing the calcium overloading in cells.The study results also suggest that "XNKQ" acupuncture method can regulate the[Ca2+]i in the focal cerebral is chemia,and the earlier the better.All of these offer the evidence to use acupuncture at early time in clinic. |
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