|
| Expression of TGF-β1 and CTGF in rats with experimental diabetic macroangiopathy and intervention role of traditional Chinese medicine |
| Hits 2373 Download times 2229 Received:April 22, 2012 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2012.03.22 |
| Key Words
diabetes mellitus 2 macroangiopathy;macroangiopathy fibrosis;traditional Chinese medicine;CTGF;TGF-β1;immunohistochemistry |
| Author Name | Affiliation | | WANG Jun | The First Hospital Affiliated of Tianjin University of TCM, Tianjin 300193, China | | XU Yang | The First Hospital Affiliated of Tianjin University of TCM, Tianjin 300193, China | | YUAN Xiang-ke | The First Hospital Affiliated of Tianjin University of TCM, Tianjin 300193, China | | ZHANG Xue-yong | The First Hospital Affiliated of Tianjin University of TCM, Tianjin 300193, China | | DING Zhi-ming | The First Hospital Affiliated of Tianjin University of TCM, Tianjin 300193, China |
|
| Abstract
|
| [Objective] To explore the pathogenesis mechanism of macroangiopathy in diabetic patients trough observing the expression of TGF-β1 and CTGF in rats with experimental diabetic macroangiopathy and decide the target in preventing and treating the macroangiopathy in patients with diabetes through observing the preventing effect of TCM. [Methods] Among eighty male SD rats seventy were induced with diabetes (DM). They were divided into 5 groups: model group (n=14), high, medium and low dose of traditional Chinese medicine groups (n=14), melbine group (n=14). All rats in these six groups were giving with ordinary feed for 12 weeks. After that, arteria cruralis was obtained and the site and change of expression of CTGF and TGF-β1 in which were observed. [Results] TGF-β1 was mainly expressed in the cytoplasm. It is strong positive in model group. In Chinese medicine intervention groups the expression of TGF-β1 was declined obviously (P<0.01). Melbine has influence on the expression of TGF-β1, but the comparison with high dose of traditional Chinese medicine group has significant difference (P<0.05). A large of brown-1yellow material with strong positive reaction (CTGF) could be seen in cytochylema in submucosa of blood vessels of model group. But the CTGF expression was all significantly lowered in cell of blood vessels in drug intervention groups (P<0.01). The difference of expression between high dose group and Western medicine control group was statistically different. [Conclusion] 1)The onset of diabetic angiopathy may be concerned with fibrosis. 2)Modified Taohe Chengqi Decoction can reduce the expression of Fibrosis factor TGF-β1 and CTGF in rats with experimental diabetic macroangiopathy. It can improve macroangiopathy in DM and has a dose-1dependent relationship. |
|
|
|
|
|
|
|
