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| Reproducing chronic ulcerative colitis in rats and its procedure conformity |
| Hits 2542 Download times 2780 Received:January 05, 2012 |
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| DOI
10.11656/j.issn.1672-1519.2012.03.23 |
| Key Words
2, 4, 6-trinitrobenzenesulfonic acid;dextran sulfate sodium;chronic ulcerative colitis;animal model |
| Author Name | Affiliation | | SUN Xiao-ping | TCM Department, General Hospital of Tianjin Medical University, Tianjin 300052, China | | HOU Li-juan | TCM Department, General Hospital of Tianjin Medical University, Tianjin 300052, China | | WANG Xiao-hong | TCM Department, General Hospital of Tianjin Medical University, Tianjin 300052, China | | TANG Fang | TCM Department, General Hospital of Tianjin Medical University, Tianjin 300052, China |
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| Abstract
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| [Objective] To reproduce chronic ulcerative colitis (UC) in rats and establish its procedure conformity. [Methods] Male Wistar rats were randomly divided into normal control group and model group. Acute colitis was induced by rectal administration of 2, 4, 6-trinitrobenzenesulfonic acid solution (TNBS) dissolved in 50% ethanol, 100 mg/kg. Ten days later, when the stool occult blood of rats showed “1+”, ten model rats were randomly selected and killed to observe colon histological changes; the other model rats were randomly divided into positive (PC) group and (UC) group. The occurrence of colitis was induced with drinking 5% dextran sulfate sodium (DSS) solution or normal saline. After modeling the disease activity index (DAI) was observed in each group. The colonic mucosal damage index (CMDI) and the histological changes were assessed et the end on experiment. [Results] Compared with normal group and positive group, DAI and CMDI scores of chronic ulcerative colitis group were significantly increased (P<0.05); the histological changes were also differed significantly between them. [Conclusion] The chronic ulcerative colitis was successfully reproduced in rats. The method of using TNBS/alcohol solution to induce inflammation first and using DDS to induce its recurrence can imitate the characteristics of replaced onset of acute and chronic stage in UC group. It has similar pathological changes to that in human body. |
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