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Technical study on microwave-assisted extraction from Radix Bupleuri
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DOI   10.11656/j.issn.1672-1519.2012.03.28
Key Words   Bupleurum Chinese DC.;saikosaponin a、d;microwave-assisted extraction
Author NameAffiliation
LI Di-hua Department of Medical Research, Tianjin Institute of Acute Abolominal Diseases of Integrated Traditional Chinese and Westen Medicine, Tianjin 300100, China 
LIU Jun-hong Department of Medical Research, Tianjin Institute of Acute Abolominal Diseases of Integrated Traditional Chinese and Westen Medicine, Tianjin 300100, China 
WU Xiao-xian Department of Medical Research, Tianjin Institute of Acute Abolominal Diseases of Integrated Traditional Chinese and Westen Medicine, Tianjin 300100, China 
LV Yuan-shan Department of Medical Research, Tianjin Institute of Acute Abolominal Diseases of Integrated Traditional Chinese and Westen Medicine, Tianjin 300100, China 
Abstract
    [Objective] To optimize the technological conditions for the exaction of effective compounds from Bupleurum Chinese DC. by microwave-assisted art. [Methods] Taking the results of the routine backflow extraction optimized by an orthogonal test as the standard and using HPLC for monitoring the contents of saikosaponin the influence factors for microwave-assisting extraction from Radix Bulpleuri were optimized. [Results] The optimized extractions conditions were: to set the microwave power rate at 800 W; to exact three times with eight times volume of ethyl alcohol for 20 min each time. [Conclusion] The technique with microwave extraction is better than the method of backflow extraction with high efficiency and short extraction time.

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