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| Effect of acupuncture at Neiguan acupoint on the gene expression profile of rats with acute myocardial ischemia |
| Hits 1712 Download times 1694 Received:April 26, 2012 |
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| DOI
10.11656/j.issn.1672-1519.2012.04.14 |
| Key Words
acupuncture;acute myocardium ischemia;expression of gene function |
| Author Name | Affiliation | | LIANG Xian-ru | Affiliated Hospital of Logistics College of Chinese People's Armed Police Troop, Tianjin 300262, China | | XI Qiang | Tianjin University of TCM, Tianjin 300193, China | | LI Xiao-mei | Tianjin University of TCM, Tianjin 300193, China | | CUI Rui | Tianjin University of TCM, Tianjin 300193, China | | JIN Guang | Tianjin University of TCM, Tianjin 300193, China | | GUO Yi | Tianjin University of TCM, Tianjin 300193, China | | GUO Yong-ming | Tianjin University of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] To study the effect of acupuncture on the gene expression profile in acute myocardial ischemia. [Methods] Aacute myocardial ischemic was induced by ligating the left anterior descending branch of coronary artery, and the total RNA of the ischemic myocardium was extracted after grouping and intervention. Afterwards, we hybridized the extraction with the whole gene expression profile chip in rat and detected them. In the differential expressed gene during therapeutic process after screening, we searched and analyzed the compound functional classification of the significant aggregative differential expression by the means of gene functional classification system and discussed the effect of acupuncture on the gene expression profile in acute myocardial ischemia from the level of gene function. [Results] The expression of 398 genes was up-regulated after 24 hours of myocardial ischemia. However, the expression of 298 gene (74.87% of the total up-regulated genes) showed the tendency of down-regulation after acupuncture at Neiguan acupoint three times intermittently; in the 338 down-regulated genes after 24 hours of myocardial ischemia, the expression of 188 genes (55.62% of the total down-regulated genes) showed the tendency of up-regulation after acupuncture at Neiguan acupoint three times intermittently, indicating a benign regulative action of the expression of ischemia myocardium gene after acupuncture at Neiguan acupoint. In the screened differential expression genes, the functional module of Ppm1b, Suclg1, Got2, Mdhl, PDP1, Fh, Cpb2, Ywhaz, S100A9, Atp1b1, TnI, Myl3, Casq2, Mb, mainly involved the oxidative phosphorylation, the electron transfer of mitochodria, the electron transfer coupling of ATP synthesis, the energy metabolism, the process of phoshates metabolism, the univalent inorganic cation transfer, the process of coenzyme metabolism and so on. [Conclusion] Acupuncture at Neiguan acupoint may protect the myocardium by influencing the genes mentioned above. |
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