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| Effect of Gengnian Pingtiao liquid on expression of Bcl-2 and Bax in myocardial cell of rats with climacteric |
| Hits 1347 Download times 1459 Received:February 13, 2012 |
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| DOI
10.11656/j.issn.1672-1519.2012.04.20 |
| Key Words
Gengnian Pingtiao liquid;cardiac muscle;menopause rats;Bcl-2;Bax;apoptosis;immunohistochemistry |
| Author Name | Affiliation | | JIANG Bo | Department of TCM, The Third Hospital of Wuhan, Wuhan 430061, China | | ZHANG Duan-lian | Basic Medical College of Wuhan University, Wuhan 430062, China |
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| Abstract
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| [Objective] To explore of protective effects of Gengnian Pingtiao liquid on myocardial cell of rats with Climacteric. [Methods] The 20 SD rats of 11 to 15 months old with the natural climacteric were randomly divided into two groups: Gengnian Pingtiao liquid group (group P), menopause control group (group M). Another 10 SD rats, 4~6 month old were taken for young control group (group Y). After experiment for 45 days, the average optical density(OD) and the rate of positive area of expression of Bcl-2 and Bax were analyzed by image analysis with HPIAS-2000 analysis system. [Results] The expression of Bcl-2 in group P was significantly higher than that in group M (P < 0.01). No significant difference of expression of Bcl-2 was found between the group P and that in group Y (P ＞ 0.05). The expression of Bax in group P was significantly lower than that in group M (P < 0.01). No significant difference was found in the expression of Bax between group P and that in group Y (P ＞ 0.05). [Conclusion] Gengnian Pingtiao liquid can inhibit the myocardial cell apoptosis and plays an important protective role for myocardial cell. |
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