|
| Experimental study on isolation, culture and identification of MSCs in rats |
| Hits 1896 Download times 2163 Received:June 15, 2012 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2012.05.18 |
| Key Words
Bone marrow mesenchymal stem cell;density gradient centrifugation method;isolation in vitro;identification |
| Author Name | Affiliation | | CUI Jie | Tianjin University of TCM, Tianjin 300193, China | | FAN Ying-chang | Tianjin University of TCM, Tianjin 300193, China | | XUE Liang | Tianjin University of TCM, Tianjin 300193, China |
|
| Abstract
|
| [Objective] To isolate, culture and purify bone marrow mesenchymal stem cells(MSCs). [Methods] MSCs from the rat bone marrow was obtained and purified in vitro by the method of gradient centrifugation combining with adherence culture. They were assess by immunocytochemistry. [Results] The samples of MSCs of third passage could express CD44, but not CD34, according to the characteristics of the MSCs. [Conclusion] The method of density gradient centrifugation can establish a stable system to separate and culture MSCs in vitro. |
|
