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| Effect of Shenxiaokang on the renal tissues expression of Gene Gna12 in diabetic nephropathy rats |
| Hits 1494 Download times 2328 Received:June 23, 2012 |
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| DOI
10.11656/j.issn.1672-1519.2012.05.20 |
| Key Words
Shenxiaokang;diabetes;diabetic kidney damage;gene expression profiling;Guanine nucleotide binding protein alpha 12 |
| Author Name | Affiliation | | QIAO Yu | Basic Medical College, Heilongjiang University of TCM, Harbin 150040, China | | XIE Ying | Basic Medical College, Heilongjiang University of TCM, Harbin 150040, China | | BAI Yu-bin | Basic Medical College, Heilongjiang University of TCM, Harbin 150040, China | | WANG Bing | Basic Medical College, Heilongjiang University of TCM, Harbin 150040, China | | CHEN Fei | Basic Medical College, Heilongjiang University of TCM, Harbin 150040, China | | LIU Chun-hong | Basic Medical College, Heilongjiang University of TCM, Harbin 150040, China | | LIU Cheng-gang | Basic Medical College, Heilongjiang University of TCM, Harbin 150040, China | | CHANG Jia-yi | Basic Medical College, Heilongjiang University of TCM, Harbin 150040, China | | GAO En-yu | Basic Medical College, Heilongjiang University of TCM, Harbin 150040, China | | HAN Jie-ru | Basic Medical College, Heilongjiang University of TCM, Harbin 150040, China | | JIANG De-you | Basic Medical College, Heilongjiang University of TCM, Harbin 150040, China |
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| Abstract
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| [Objective] To observe the therapeutical effect of a complex prescription of Shenxiaokang on DN rats and discuss the mechanism of action in the treatment of DN, then provide experimental data for clinical practice. [Methods] The model rats male Wistar were established with multiple injections of low dose STZ and high calorie diet. Kinds of examination methods were applied, including radiommunoassay, biochemistry, light microscope, electron microscrope, immunohistochemical, Affymetrix Co.US. rat 2302.0 gene expression profiling chip, and real time quantitative reverse transcription polymerase chain reaction technique to observe the effect of Shenxiaokang on indicators. Most especially applied cluster analysis method to make certain important associated genes with DN, and further applied RT-PCR to do confirmatory experiment. [Results] Gna12 gene was down-regulation in the DN model group rat kidneys and up-regulation in the Shenxiaokang treatment group rat kidneys. [Conclusion] Applied Affymetrix Co.US. rat 2302.0 gene expression profiling chip to test diabetic nephropathy rats renal tissues genes expresstion, Gna12 gene is one of the renal tissues difference expresstion and drug effect targets and applied real time quantitative reverse transcription polymerase chain reaction technique to analyze it. It is the first time at home and abroad. To genes function analyze show that, the Gna12 gene is down-regulation in the DN model group rat kidneys. It is important associated with diabetic kidney damage, and up-regulation in the Shenxiaokang treatment group rat kidneys. Shenxiaokang has benign effect on Gna12 gene Expresstion. Its mechanism remains to be studied further. |
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