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| Effect of gingko lactone A and gingko lactone B on cholesterol metabolism in PC12 cells under high cholesterol culture |
| Hits 1403 Download times 1779 Received:June 20, 2012 |
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| DOI
10.11656/j.issn.1672-1519.2012.05.21 |
| Key Words
Ggingko lactone A;Gingko lactone B;Cholesterol;PC12 cells;blood-brain barrier |
| Author Name | Affiliation | | ZHANG Yun-sha | Department of Pathology, Tianjin University of TCM, Tianjin 300193, China | | MA Dong-ming | Laboratory of Pathology, Tianjin University of TCM, Tianjin 300193, China | | LU Bin | Laboratory of Pathology, Tianjin University of TCM, Tianjin 300193, China | | MA Yi-wen | Maternal and Child Health Hospital of Tanggu District, Tianjin 300451, China | | JIANG Xi-juan | Department of Pathology, Tianjin University of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] To observe the cholesterol metabolism in PC12 cells with high cholesterol-induced dysfunction under in vitro blood-brain barrier (BBB)-based environment and to explore the effects of gingko lactone A and B(GKA and GKB). [Methods] The rat brain microvascular endothelial cells and astrocytes were used to establish the BBB model by co-cultured in the Transwell device with PC12 cells. Cholesterol, 40 μmol/L, was added into the Transwell endothelial cell side. GKA at a dose of 30 μmol/L and GKB at a dose of 25 μmol/L were applied to deal with the culture system respectively. After intervention, RT-PCR technique was used to detect the cholesterol metabolism key enzyme of LRP-1, ACAT, ABCA1 and CYP46. [Results] Under high cholesterol environment, the expression of LRP-1 decreased (P<0.01) and the expression of ACAT and ABCA1 increased (P<0.05). After intervention, the expression of LRP-1 was increased in varying degrees (P<0.05), but no significant changes were found in other enzymes. [Conclusion] GKA and GKB can affect the expression of LRP-1 in PC12 cells. |
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