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| Simultaneous determination of chlorogenic acid, cryptochlorogenic acid and scopoletin in Artemisia Annua L. by HPLC |
| Hits 1479 Download times 1494 Received:May 31, 2012 |
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| DOI
10.11656/j.issn.1672-1519.2012.05.24 |
| Key Words
Artemisia Annua L.;HPLC;chlorogenic acid |
| Author Name | Affiliation | | SUN Yu-gang | Tianjin State Kay Laboratory of Mordern Chinese Medicine, Tianjin University of TCM, Tianjin 300193, China
Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China | | QIN Xu-wen | Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang, 222001, China | | ZHANG Ling | Tianjin State Kay Laboratory of Mordern Chinese Medicine, Tianjin University of TCM, Tianjin 300193, China | | LI Jin | Tianjin State Kay Laboratory of Mordern Chinese Medicine, Tianjin University of TCM, Tianjin 300193, China | | XIAO Wei | Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang, 222001, China | | WANG Zhen-zhong | Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang, 222001, China | | CHENG Zhi-dong | Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang, 222001, China | | CHANG Yan-xu | Tianjin State Kay Laboratory of Mordern Chinese Medicine, Tianjin University of TCM, Tianjin 300193, China
Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang, 222001, China
Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China |
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| Abstract
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| [Objective] A sensitive method of the determination of chlorogenic acid, cryptochlorogenic acid and scopoletin content in traditional Chinese medicine Artemisia annua L. was established by high performance liquid chromatography (HPLC). [Methods] The separation was achieved by use of C18 analytical column (4.6 mm×250 mm, 5 μm) at 30℃, while the injected sample volume was set to 10 μL. A constant flow rate of 1.0 mL/min was employed throughout the analysis. A binary gradient elution program of aqueous formic acid (0.1%, v/v) versus acetonitrile was selected for the quantitative analysis of chlorogenic acid, cryptochlorogenic acid and scopoletin. [Results] The HPLC method showed the satisfactory linearity, and the results of the method validation meeted the relevant requirements. The method had been successfully applied to the determination of chlorogenic acid, cryptochlorogenic acid and scopoletin content in Artemisia L. samples. [Conclusion] The established HPLC method could be used to evaluate the quality of Artemisia annua L. |
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