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| The esablishment of quality standard for Shouzukou mixture |
| Hits 1419 Download times 1602 Received:August 26, 2012 |
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| DOI
10.11656/j.issn.1672-1519.2012.05.26 |
| Key Words
Shouzukou mixture;Baicalin;TLC;HPLC |
| Author Name | Affiliation | | LIN Hong | Tianjin Children's Hospital, Tianjin 300074, China | | ZHANG Nan | Tianjin Children's Hospital, Tianjin 300074, China | | CHEN Shuang-lu | Tianjin Children's Hospital, Tianjin 300074, China | | QIAN Yu-kun | Tianjin Children's Hospital, Tianjin 300074, China |
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| Abstract
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| [Objective] To esablish the quality standard for Shouzukou Mixture.[Methods] Scutellaria baicalensis, Coptis Chinensis, Golden cypress, Cape jasmine, dandelion were identificated by TLC; chlorogenic acid in Shouzukou Mixture was determinated by HPLC. Parameters: Shim-pack VP-ODS (4.6 mm×150 mm), methyl alcohol: water:glacial acetic acid (40:60:1); gradient elution;a flow rate of 1.0 mL/min; detection wavelength of 280 nm; column temperature 40℃。 [Results] All five tranditional Chinese medicine could be checked by using TLC; HPLC determination of baicalin in the range of 0.270～0.720 μg was good linear with a good relationship. Average recovery was 100.8%. [Conclusion] The method is simple, accurate and reliable, and suitable for the quality control. |
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