Key Words
metabolomics;comprehensive analysis;transporter;organic anion
Author Name
Affiliation
Yoshikatsu Kanai
Japan
HE Xin
Tianjin University of TCM, Tianjin 300193, China
Abstract
Transporters are membrane proteins that allow efficient and selective permeation of organic and inorganic solutes through the membrane. The molecular identification of transporter genes has facilitated the understanding of functions of transporter proteins by in vitro experimental approaches in which cloned cDNAs are expressed in exogenous expression systems. The metabolomics analysis to compare chemical compounds in urine, blood or tissue samples quantitatively between wild type and transporter-knockout mice gives us clues to the real substrates of the transporter in vivo. This approach can also be used to find out functions of orphan transporters. For example, we have applied metabolomics with capillary electrophoresis-mass spectrometry (CE-MS) to reveal the function of an orphan transporter in SLC22 transporter family.
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