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| Isolation of phenolic constituents from Picrorhiza scrophulariiflora Pennell and UPLC-ESI-MS analysis |
| Hits 2482 Download times 1994 Received:June 13, 2012 |
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| DOI
10.11656/j.issn.1672-1519.2012.06.21 |
| Key Words
Picrorhiza scrophulariiflora Pennell;UPLC-ESI-MS;Phenolic constituents;isolation |
| Author Name | Affiliation | | LIU Shi-qiao | Tianjin University of TCM, Tianjin 300193, China | | ZHANG Xin-xin | Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China | | SHAN Qi | Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China | | ZHOU Fu-jun | Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China | | HOU Wen-bin | Tianjin University of TCM, Tianjin 300193, China
Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China |
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| Abstract
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| [Objective] To study the Phenolic constituents in Picrorhiza scrophulariiflora Pennell and determine their contents in the medicinal materials. [Methods] The compounds were isolated by the solvent extract, silica gel and Sephadex LH-20 columns and their structure were elucidated by 1H-NMR、13C-NMR and UPLC-ESI-MS which were used as analysis standards. Their contents in the extract and medicinal materials were determined by UPLC-ESI-MS. [Results] Six phenolic compounds were obtained and identified as Picein (Ⅰ), Androsin (Ⅱ), Scrophenoside A(Ⅲ), Scrophenoside F(Ⅳ), Vanillic acid (Ⅴ) and Acetovanillone(Ⅵ). [Conclusion] The contents of above compounds in the extract are 1.50%, 5.62%, 3.53%, 1.96%, 0.78% and 3.16%, and the contents in the medicinal materials are 0.24%, 0.90%, 0.56%, 0.31%, 0.13%, 0.51%, respectively. |
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