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| Influence of the serum of SCI rats after fire needle intervention on the proliferation and differentiation of neural stem cells |
| Hits 2089 Download times 1592 Received:December 15, 2012 |
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| DOI
10.11656/j.issn.1672-1519.2013.01.09 |
| Key Words
spinal cord injury;fire needle/acupuncture;neural stem cell;proliferation and differentiation |
| Author Name | Affiliation | | LI Yan | Tianjin Public Security Hospital, Tianjin 300042, China | | ZHOU Zhen | The Second Hospital Affiliated of Tianjin University of TCM, Tianjin 300150, China | | CHENG Su-li | Tianjin University of TCM, Tianjin 300193, China | | JIAO Zhao-hua | Tianjin University of TCM, Tianjin 300193, China | | CHEN Shuang | Tianjin University of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] To study the influence of the extracted serum of SCI rats after fire needle intervention on the proliferation and differentiation of neural stem cells in vitro. [Methods] Fifteen SD rats were randomly divided into sham operation, model and fire-needle groups, and the serum was extracted in each group after 3 days' corresponding intervention. The neural stem cells were separated from the spinal cord tissue in Kunming mice embryo after 14 days' pregnancy, and then cultured by all the above serum in each group to observe the proliferation and differentiation states of neural stem cells 7 days later. [Results] Neural stem cells of each group could survive, proliferate and differentiate into neurons. The proliferation number of neural stem cells in fire needle group was obviously more than that in sham operation and model group, and the percentage of differentiation into neurons in fire group was higher than that in the other groups (P<0.05). [Conclusions] The serum of SCI rats after fire needle intervention could promote the proliferation and differentiation into neurons of neural stem cells. |
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