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| Discussion on evaluating criteria of model of middle cerebral artery occlusion in rats |
| Hits 1729 Download times 1556 Received:September 12, 2012 |
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| DOI
10.11656/j.issn.1672-1519.2013.01.12 |
| Key Words
cerebral ischemia model;model evaluation standards;Laser Doppler cerebral blood flow measurement |
| Author Name | Affiliation | | CUI Jing-jun | Tianjin University of TCM, Tianjin 300193, China | | HE Jiao-jun | Tianjin University of TCM, Tianjin 300193, China | | LI Jing | The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China | | DU Yuan-hao | The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] To choose criteria of suitable evaluation and enrolling and excepting method for improving the judgment accuracy of the cerebral ischemia model. [Methods] Adopting suture-occluded method the model of middle cerebral artery occlusion (MCAO) was established in Wistar ratsl. The change of rCBF was measured with Laser Doppler Blood Flow assessment. The neurological state was assessed by Zea-Longa neurological deficit scores at 1, 2, 3, 6, 9, 12, 15, 18, 21 and 24 h after cerebral infarction; then the samples were prepared and Triphenyltetrazolium chloride (TTC) staining was used to evaluate the infarct condition. [Results] Anatomic examination and TTC staining showed that neurobehavioral scoring method can correctly judge for 44 rats with MCAO and the accuracy rate was 75.86%. When combined with the result of the anatomic examination, the accuracy rate was rised to 86.21%. The measure of rCBF with Laser Doppler blood flow measurement was accurate in 51 rats, and its accuracy rate was 87.93%. When combined with the result of anatomic examination, the accuracy rate will be 100%. [Conclusion] Measuring the changing of rCBF with Laser Doppler blood flow assessment is a sensitive and accurate method to evaluate the MCAO in rats, especially used in the super-acute phase. |
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