|
| Effect of tenuigenin on O-GlcNAc glycosylation and phosphorylation of tau protein in PC12 cells |
| Hits 2179 Download times 1341 Received:November 10, 2012 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2013.01.14 |
| Key Words
tenuigenin;PC12 cell;tau protein;O-GlcNAc glycosylation;phosphorylation |
| Author Name | Affiliation | | CHEN Yu-jing | Xuanwu Hospital, Capital Medical University, Beijing 100053, China | | HUANG Xiao-bo | Xuanwu Hospital, Capital Medical University, Beijing 100053, China | | CHEN Wen-qiang | Xuanwu Hospital, Capital Medical University, Beijing 100053, China | | WANG Ning-qun | Xuanwu Hospital, Capital Medical University, Beijing 100053, China |
|
| Abstract
|
| [Objective] To study the effect of tenuigenin on O-GlcNAc glycosylation and phosphorylation of tau protein in PC12 cells. [Methods] The level of O-GlcNAcylation in differenciated PC12 cells was modulated by changing the concentration of the sugar in culture medium. Then, the consequent changes of tau phosphorylation at various phosphorylation sites were examined by using Western blot. MTT method was used for the detection of cell survival rate. [Results] Tenuigenin could significantly improve the survival rate of PC12 cells. Compared with the control group, the expression of O-GlcNAc glycosylation in model group decreased, and phosphorylation of tau protein increased. Tenuigenin could increase the expression of O-GlcNAc glycosylation and decrease the expression of phosphorylation of tau protein. [Conclusion] These results suggest that O- GlcNAcylation negatively modulates tau phosphorylation at some phosphorylation sites, and tenuigenin could increase O-GlcNAc expression and decrease phosphorylation of tau protein. |
|
|
|
|
|
|
|
