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| Content determination of polysaccharides in Radix Glycyrrhizae from three different species |
| Hits 1810 Download times 2308 Received:September 04, 2012 |
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| DOI
10.11656/j.issn.1672-1519.2013.01.16 |
| Key Words
Radix Glycyrrhizae polysaccharides;Phenol-sulfuric acid colorimetry method;quantitative determination |
| Author Name | Affiliation | E-mail | | LI Wei | Department of Pharmacy, No. 323 Hospital of PLA, Xi'an 710054, China | | | SONG Xin-bo | Tianjin Zhongyi pharmaceutical Co., Ltd., Tianjin 300193, China | songxinbo@tjutcm.edu.cn | | SUN Cheng-rong | Department of Pharmacy, No. 323 Hospital of PLA, Xi'an 710054, China | | | XIA Qing | Department of Pharmacy, No. 323 Hospital of PLA, Xi'an 710054, China | |
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| Abstract
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| [Objective] An phenol-H2SO4 colorimetry method was developed for the determination of polysaccharides in Radix Glycyrrhizae samples from different species in order to provide a scientific basis for a quality evaluation of Materia Medica. [Methods] The samples were extracted with 95% ethanol to remove the little polarity interference components during the pretreatment process, then water extraction and alcohol precipitation were performed, then the obtained polysaccharides were refined by passing many processes. A corrected factor was used to minimize the error between determinations of glucose and polysaccharides. The contents of the polysaccharides in Radix Glycyrrhizae of different samples were determined by phenol-H2SO4 colorimetry method. [Results] The content of three different species' raw material polysaccharides was Radix Glycyrrhizae Glabrae >Radix Glycyrrhizae Inflatae >Glycyrrhiza uralensis. The average recovery rate for the Glycyrrhiza uralensis polysaccharides was 98.23% with 1.63% of RSD (n=6). [Conclusion] This determination method is simple, practical, with good reproducibility, high average recovery rate and the color of the treated samples are stabilized within 4h. So this method can be used to determine of Radix Glycyrrhizae polysaccharides. |
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