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| Determination of rutin content in Gynura divaricata by HPLC |
| Hits 2269 Download times 1358 Received:December 03, 2012 |
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| DOI
10.11656/j.issn.1672-1519.2013.02.18 |
| Key Words
Gynura divaricata;HPLC;rutin;assaying |
| Author Name | Affiliation | | WANG Zhong-hua | The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China | | CHEN Tao | The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] To establish assay method about the main ingredients of rutin in Gynura divaricata with HPLC. [Methods] Pretreatment was performed by petroleum ether (30~60℃). Mobile phase was “methanol-1% glacial acetic acid (40:60)”. The detection wavelength was 360 nm. Sample volume was 40 mL 1.000g 60% ethanol extract. Ultra extract was processed for 15 min. Chromatographic column was Xterra @RP 185 um. [Results] According to the linear relationship, it was showed that linear relation of rutin mass concentration was good in 0.011 2~0.067 2 mg/mL, r=0.999 1. All accuracy, reproducibility, stability and recovery could meet the analysis demand. [Conclusion] This method is simple and accurate, and it can provide some certain references for determination of rutin content in Gynura divaricata. |
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