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| Influence of Shufeng Tongluo prescription on expression of VCAM-1/PI3K/Rac-1/NOX2/NOX4/SHP-2 related factors in eosinophils transendothelia migration in lung of rats with asthma |
| Hits 1954 Download times 1647 Received:December 24, 2012 |
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| DOI
10.11656/j.issn.1672-1519.2013.03.13 |
| Key Words
asthma;eosinophils;transmembrane migration;Shufeng Tongluo prescription;gene expression |
| Author Name | Affiliation | E-mail | | LIU Gui-ying | Respiratory Department, The First Affilated Hospital of Tianjin University of TCM, Tianjin 300193, China | | | ZHANG Hui-qi | Respiratory Department, The First Affilated Hospital of Tianjin University of TCM, Tianjin 300193, China | huiqizhang2006@163.com | | YIN Xin-zhong | Respiratory Department, The First Affilated Hospital of Tianjin University of TCM, Tianjin 300193, China | | | ZHU Zhen-gang | Respiratory Department, The First Affilated Hospital of Tianjin University of TCM, Tianjin 300193, China | | | LIU Min | Respiratory Department, The First Affilated Hospital of Tianjin University of TCM, Tianjin 300193, China | | | QIAO Ya-zhen | Respiratory Department, The First Affilated Hospital of Tianjin University of TCM, Tianjin 300193, China | | | DOU Ying-ting | Respiratory Department, The First Affilated Hospital of Tianjin University of TCM, Tianjin 300193, China | | | LIU Wen-bin | Respiratory Department, The First Affilated Hospital of Tianjin University of TCM, Tianjin 300193, China | | | LIU Chao-wu | Respiratory Department, The First Affilated Hospital of Tianjin University of TCM, Tianjin 300193, China | | | DI Guan-lin | Respiratory Department, The First Affilated Hospital of Tianjin University of TCM, Tianjin 300193, China | | | CHANG Li | Respiratory Department, The First Affilated Hospital of Tianjin University of TCM, Tianjin 300193, China | | | TIAN Chang-hong | Respiratory Department, The First Affilated Hospital of Tianjin University of TCM, Tianjin 300193, China | |
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| Abstract
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| [Objective] To explore the effect of Shufeng Tongluo decoction on eosinophils transendothelia migration in lung of rats with asthma. [Methods] Seventy Wistar rats (SPF) were randomly divided into seven groups: normal group, model group, dexamethasone group, DPI group, low-dose group of Chinese medicine, medium-dose group of Chinese medicine, and high-dose group of Chinese medicine with 10 rats in each group. The rats in normal group were sensitized by 1 mL of intra-peritoneal injection of normal saline. The other 6 groups were given with 1 mL sensitization liquid, contained 100 mg OVA (Grade V), 100 mg aluminum hydroxide and 6×109 inactivated whooping cough bacili on the first day of experimental study. From 15th day, the rats in normal group were administrated with 1 mL of normal saline orally, 2 hours later the rats were challenged by 6 mL normal saline in a box of aerosol inhalation once a day for 30 min a time and keeping for 10 days; the rats in other 6 group were respectively administrated with corresponding drugs orally and 2 hours later were challenged by 6 mL OVA (5%, V Grade) of aerosol inhalation once a day, for 30 min a time and keeping for 10 days, And 10 days later all rats were killed. The samples from rats were collected for examination. [Results] The expression of VCAM-1/PI3K/Rac-1/NOX2/NOX4/SHP-2 related factors to eosinophils transendothelia migration in lung of rat with asthma were significantly higher than that of normal group rats(P<0.001). Compared with the model group, dexamethasone group could significantly inhibit the expression of above factors(P<0.001). The effect of high-dose group of Chinese medicine was similar to dexamethasone group(P>0.05), the other treatment groups (DPI group, low-dose group of Chinese medicine and medium-dose group of Chinese medicine ) only had general inhibition effect. [Conclusion] Shufeng Tongluo decoction has some certain suppression effect on eosinophils transendothelia migration in lung of rats with asthma to a certain extent. It may be one of its action mechanisms in controlling asthma. |
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