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| Effect of Rongchang capsule on learning memory and hippocampal mossy fiber sprouting in rats with epilepsy induced by pentylenetetrazole |
| Hits 2257 Download times 1687 Received:February 05, 2013 |
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| DOI
10.11656/j.issn.1672-1519.2013.05.12 |
| Key Words
epilepsy;Rongchang capsule;learning and memory capacity;morris water maze;mossy fiber sprouting |
| Author Name | Affiliation | | YANG Chang-quan | Department of Pediatrics, The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China | | MA Rong | Department of Pediatrics, The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China | | LIANG Qian | Tianjin University of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] To explore the effect and mechanism of Rongchang capsule (RCC capsule) on learning and memory and the mossy fiber sprouting (MFS )of hippocampus in rats with epilepsy. [Methods] The 48 rats were randomly divided into normal group, model group, valproic acid sodium(VPA) group and RCC Capsule low, middle, high dosage group. To establish the model of epileptic rats kindled by PTZ. All test rats were treated for 28 days. The water maze test was assigned to evaluate RCC capsule effects on learning and memory capacity of rats. By Timm vulcanization silver staining method to observe hippocampal moss fiber budding and hippocampal structure damage were observed. [Results] Every treated group can reduce the rat attack degree water maze experiment results of RCC groups showed escape latency and number of cross platform in each group were shorter than that of the model group (P<0.05). Timm dying results show that the total area of MFS of every treatment group in CA3 hippocampus beginning layer and the dentate gyrus Timm dyeing particles molecular layer less than the model group (P<0.01). [Conclusion] RCC capsule can effectively control the attack frequency and level of the epilepsy, and improve the ability of learning and memory of epilepsy rats. It might inhibit MFS, then protect the hippocampal neurons to improve learning and memory capacity. |
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