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| Determination of icariin content in Yishen granules by HPLC |
| Hits 2335 Download times 1507 Received:February 15, 2013 |
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| DOI
10.11656/j.issn.1672-1519.2013.06.15 |
| Key Words
Yishen granule;icariin;HPLC |
| Author Name | Affiliation | | LIU Ya-qin | The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China | | LI Feng-li | The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China | | XUE Kun | The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] To establish a method to determine the content of icariin in Yishen granules. [Methods] Using the method of HPLC, the chromatographic column was the United States Waters C18 column (250 mm × 4.6 mm, 10 μm). The mobile phase was methyl cyanides-0.1% phosphoric acid solution (28:72). Velocity of flow rate was 1 mL/min. The detection wavelength was 270 nm, and column temperature was at room temperature. [Results] Icariin in 0.048 5~0.970 0 μg and chromatographic peak area had good linear relationship (r=0.999 9). The average recovery was 97%, RSD=0.7% (n=6). [Conclusion] The method is simple, specific, reproducible, and can be used for determination of icariin in Yishen granules. |
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