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| Effect of Niaoduqing granules on CYP450 and P-gp in vitro |
| Hits 1850 Download times 1676 Received:January 11, 2013 |
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| DOI
10.11656/j.issn.1672-1519.2013.07.16 |
| Key Words
CYP450;P-gp ATPase;IC50;Niaoduqing granule |
| Author Name | Affiliation | | LIU BO | Vrinary Surgery, The fourth Central Hospital of Tianjin, Tianjin 300140, China | | HU Bing | Tianjin Tasly Pharmoceutical Co. Ltd., Tianjin 300410, China | | LAN Ying | Department of Nephrology, The fourth Central Hospital of Tianjin, Tianjin 300140, China | | ZHAO Lian-yu | Department of Nephrology, The fourth Central Hospital of Tianjin, Tianjin 300140, China | | YANG Yun-hua | Department of Nephrology, The fourth Central Hospital of Tianjin, Tianjin 300140, China |
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| Abstract
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| [Objective] To investigate the effects of Niaoduqing (NDQ) on recombined human cytochrome P450 (CYP450) and P glycoprotein(P-gp) in vitro primarily. [Methods] 1)Using the Promega P450-GloTM Screening System and according to the fluorescence luminescence principle the IC50 of NDQ group and inhibitor group to the IC50 of recombined CYP450 was detected, comparing the IC50 of NDQ group and inhibitor group the inhibitory effect of NDQ on recombined human CYP1A2, CYP2D6, CYP3A4, CYP2C19, CYP2C9 was evaluated. 2)Using the BD ATPase Assay Kit and using luminescent technique the activity of P-gp ATPase in the NDQ group and blank control group was detected and through the comparison of the ATPase activity between NDQ and blank control group it was evaluated that whether NDQ was the substrates or inhibitors of recombined human P-gp or not. [Results] 1)IC50 value(g/L)in NDQ group and inhibitors group was as follows, CYP1A2：17.47, 3.37×10-6;CYP3A4：33, 4.181×10-5; CYP2C9：10.39, 1.056×10-3;CYP2D6：13.98, 2.245 9×10-6; CYP2C19：9.251, 1.442×10-3. 2)The vbg htyu78 of P-gp ATPase activity in NDQ group was 16.39 nmol/(mg·min). ATPase activity in the blank control group was 4.72 nmol/(mg·min). [Conclusion] 1)IC50 in NDQ group is greater than the corresponding inhibitor group. NDQ basically has no inhibitory effect on recombined human CYP1A2, CYP2D6, CYP3A4, CYP2C19, CYP2C9. 2)The activity of ATPase in NDQ group is higher than blank group significantly, and NDQ possibly is the inhibitor or the substrates of the recombined human P-gp. |
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