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| Study on embryo toxicity of scutellaria using safety evaluation system of traditional Chinese medicine in pregnancy |
| Hits 1784 Download times 1469 Received:March 11, 2013 |
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| DOI
10.11656/j.issn.1672-1519.2013.07.17 |
| Key Words
using of drugs in pregnancy;Chinese medicine;embryo toxicity;scutellaria |
| Author Name | Affiliation | E-mail | | ZHANG Wei | Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Tianjin University TCM, Tianjin 300150, China
Tianjin University of TCM, Tianjin 300193, China | | | SONG Dian-rong | Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Tianjin University TCM, Tianjin 300150, China | songdr58@126.com | | WANG Ya-nan | Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Tianjin University TCM, Tianjin 300150, China | | | GUO Jie | Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Tianjin University TCM, Tianjin 300150, China | |
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| Abstract
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| [Objective] To assess the embryotoxicity of scutellaria and to confirm the objectivity of evaluation system of traditional Chinese medicine in pregnancy, taking evaluation system of traditional Chinese medicine in pregnancy, using embryonic stem cell test (EST) in vitro and through the comprehensive evaluation of traditional Chinese medicine, drug-containing serum and embryo volume fractions.[Methods] D3 cell line of EST(ES-D3) and embryo fibroblast cell (Balb/c 3T3) were cultured respectively with different concentration of scutellaria and 20% scutellaria containing serum. The cell morphology was observed with phase contrast microscope and the absorbance of the resulted coloured solution was measured at 570 nm in a microplate reader. According to the concentration-effect curve, 50% inhibition of growth was obtained and the viability of ES-D3 cells and 3T3 cells was demonstrated (IC50D3 and IC503T3). Using hanging-drop-suspension-attachment method ES cells were cultured in different concentration of scutellaria for proliferation to myocardial cell. Then the expression of myosin heavy chains genes (β-MHC) was detected and the concentration of scutellaria, which could inhibit the ES proliferation for 50% was calculated ID50D3). Using a biologic statistic formula the embryo toxicity of scutellaria and it-containing serum was predicted. [Results] The half-maximal proliferation-inhibition concentration (IC50) of scutellaria on ES cell line was as follows: IC50D3, IC503T3 and ID503D3 were 25.58, 42.20 and 0.28 μg/mL respectively. It was predicted that they had heavy embryo toxicity. All low and high doses of scutellaria-containing serum presented inhibitory effect for proliferation on 3T3 and ES cell. The expression of differentiation of ES cells into β-MHC of myocardial cells was 32.5% of blank serum. [Conclusion] Scutellaria has embryo toxicity. The comprehensive evaluation system using traditional Chinese medicine-containing serum can objectively and accurately evaluate the embryotoxicity of traditional Chinese medicine. |
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