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| Mechanismof Zidian decoction for inhibiting platelet destruction in ITP in mouse |
| Hits 1873 Download times 1635 Received:April 28, 2013 |
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| DOI
10.11656/j.issn.1672-1519.2013.08.15 |
| Key Words
Zidian decoction;idiopathic thrombocytopenic purpura;macrophage;apoptosis |
| Author Name | Affiliation | E-mail | | LI Yang | Liaoning University of TCM, Shenyang 110032, China | | | QU Dao-wei | Liaoning University of TCM, Shenyang 110032, China | | | LIN Shu-ru | Liaoning University of TCM, Shenyang 110032, China | | | HAO Hong-dang | Liaoning University of TCM, Shenyang 110032, China | | | FAN Ying | Liaoning University of TCM, Shenyang 110032, China | lnzyfy@126.com |
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| Abstract
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| [Objective] To observe the effects of Zidian decoction on apoptosis and microstructure of macrophage (MΦ) in spleen in ITP in mouse and to explore the mechanism of action of the prescription in the clinical use.[Methods] The 48 BALB/C mice were randomly divided into 4 groups: the normal group,model group,prednisone acetate group-Zidian decoction group.ITP was induced with intraperitoneal injection of GP-APS (guinea pig anti-mouse platelet serum) by immunoassay in mice.We observed the microscopic structure and apoptosis of peripheral platelet and spleen macrophages.[Results] After treatment-platelet count in the treatment group increased, there was a significant difference when compared with before treatment; after treatment, MΦ apoptosis rate was increased in Zidian decoction group compared with the model group(P<0.05) and the therapeutic effect was superior to prednisone.[Conclusion] Zidian decoction has therapeutic effect by improving the microstructure of spleen macrophages in ITP and promoting macrophage apoptosis in mice. |
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