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| Effect of total anthraquinone fromZhushaqi on expression of apoptosis gene in S180-bearing mice |
| Hits 2774 Download times 1806 Received:May 20, 2013 |
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| DOI
10.11656/j.issn.1672-1519.2013.10.11 |
| Key Words
anthraquinone from Zhushaqi;S180 tumor;apoptosis gene;c-myc gene;Bcl-2 gene;p53 gene |
| Author Name | Affiliation | | ZHAO Qin | Shanxi College of TCM, Xianyang 712046, China
Tibet National College, Xianyang 712082, China | | WEI Hao | Shanxi College of TCM, Xianyang 712046, China | | HU Rui | Shanxi College of TCM, Xianyang 712046, China | | LV Teng | Shanxi College of TCM, Xianyang 712046, China | | SUN Fang-yun | Tibet National College, Xianyang 712082, China |
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| Abstract
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| [Objective] To study the effect of total anthraquinone from Zhushaqi(ZSQ) on c-myc, Bcl-2 and p53 mRNA expression in S180 tumour-bearing mice. [Methods] The S180 mice model was established. The mice were divided into 4 groups: blank control, positive control with cyclophosphamide and ZSQ in high or low dose. Ten days after continuous treatment, all the mice were sacrificed and the tumor tissue was collected. Real-time PCR was used to determine the effect of ZSQ on the expression of c-myc, Bcl-2 and p53 mRNA. [Results] ZSQ could significantly reduce the expression of c-myc, Bcl-2 and p53 mRNA expression in S180 tumor tissue. The average levels of c-myc, Bcl-2 and p53 expression in low-dose and high-dose groups of ZSQ had significantly difference to that in control group(P<0.01 or P<0.05). [Conclusion] Down-regulating the c-myc, Bcl-2 and p53 mRNA expression is probably one of the antitumor mechanisms of ZSQ. |
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