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| The influences of Phryma Leptostachya L. var. asiatica Hara extract on apoptosis and apoptosis-related factors of human hepatocellular carcinoma SMMC-7721 cells |
| Hits 2824 Download times 2100 Received:March 10, 2013 |
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| DOI
10.11656/j.issn.1672-1519.2013.10.12 |
| Key Words
Phryma leptostachya L.var.asiatica Hara extract;SMMC-7721 cell;apoptosis;Caspase-9 |
| Author Name | Affiliation | E-mail | | LI Ping | The department of internal medicine, The people's hospital of Tumen City, Tumen 133100, China | | | ZENG Chang-Qian | Medical College of Dalian University, Dalian 116622, China | zengchangqian@163.com |
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| Abstract
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| [Objective] To observe apoptosis of human hepatocellular carcinoma SMMC-7721 cells induced by Phryma Leptostachya L. var. asiatica Hara extract and to investigate the related molecular mechanism. [Methods] Flow cytometry was used to detect the apoptosis rate of SMMC-7721 cells induced by Phryma Leptostachya L.var. asiatica Hara extract. Immunohistochemistry technique was used to determine the expressions of caspase-9、-3、-7 and XIAP proteins of SMMC-7721 cells. [Results] The apoptotic rate of SMMC-7721 cells treated with 40.91 μg/mL Phryma Leptostachya L. var. asiatica Hara extract was(26.84±1.23)% and increased obviously than control group(P<0.05). The expressions of Caspase-9 、-3 、-7 proteins of SMMC-7721 cells treated with 40.91 μg/mL Phryma Leptostachya L. var. asiatica Hara extract decreased than control group(P<0.05). The expression of XIAP protein of SMMC-7721 cells treated with 40.91 μg/mL Phryma Leptostachya L. var. asiatica Hara extract increased than control group(P<0.05). [Conclusion] Phryma Leptostachya L. var. asiatica Hara extract could induce the apoptosis of SMMC-7721 cells by XIAP/Caspase signal pathway. |
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