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| Study on the β-catenin expression in the process of salvianolic acid B induced differetiation of MSCs into cardiomyocytes in vitro |
| Hits 1722 Download times 1772 Received:February 03, 2013 |
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| DOI
10.11656/j.issn.1672-1519.2013.11.10 |
| Key Words
Salvianolic acid B;MSCs;cardiomyocytes;Wnt signaling |
| Author Name | Affiliation | E-mail | | JI Hong | TCM College, Tianjin University of TCM, Tianjin 300193, China | | | GAO Qing | TCM College, Tianjin University of TCM, Tianjin 300193, China | | | HU Xian-tong | TCM College, Tianjin University of TCM, Tianjin 300193, China | | | FAN Ying-chang | TCM College, Tianjin University of TCM, Tianjin 300193, China | fych@tjutcm.edu.cn |
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| Abstract
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| [Objective] To discuss the role of salvianolic acid B in inducing differention of MSCs into cardiomyocytes and the β-catenin expression in the induced MSCs.[Methods] A method has been established of culturing MSCs from rat bone marrow. The third-passage MSCs were divided into four groups: the control group, the 5-Azacytidine group, the salvianolic acid B group, the 5-Azacytidine combined with salvianolic acid B group. After induced for 4 weeks, cardiac-specific cTnT expression was detected by immunocytochemistry and the mRNA expression of β-catenin and GSK was detected by RT-PCR in each group. [Results] The expression of cTnT in each induced group was significantly higher than that in the control group. The mRNA expression of GSK in each induced group was significantly up-regulated and that of β-catenin was down-regulated when compared with control group. [Conclusion] SalB can promote the differention of cardiomyocytes from MSCs and there is a typical inhibition of the Wnt signaling in this process. |
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