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| Prevention effect analysis of the Yigan Jiangzhi prescription on D-lactic acid and diamine oxidase in SD rats with alcoholic fatty liver disease |
| Hits 2246 Download times 1944 Received:May 21, 2013 |
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| DOI
10.11656/j.issn.1672-1519.2013.12.12 |
| Key Words
Yigan Jiangzhi prescription;alcoholic fatty liver;intestinal barrier;D-lactic acid;diamine oxidase |
| Author Name | Affiliation | E-mail | | ZHAO Yuan-hong | Department of Oncology, The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China | | | YIN Ji-hong | Department of Postgraduate, Tianjin University of TCM, Tianjin 300193, China | 987129831@qq.com | | LI Zheng | Pharmacy Room, The First Affiliated Hospital of, Tianjin University of TCM, Tianjin 300193, China | lizheng@shitian.com | | YANG Pei-ying | Department of Oncology, The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China | | | LI Quan-zheng | Department of Postgraduate, Tianjin University of TCM, Tianjin 300193, China | | | YANG Cai-xia | Department of Postgraduate, Tianjin University of TCM, Tianjin 300193, China | | | HOU Shan-shan | Department of Postgraduate, Tianjin University of TCM, Tianjin 300193, China | | | ZHANG Xiao-rui | Department of Postgraduate, Tianjin University of TCM, Tianjin 300193, China | | | JIA Ying-jie | Department of Oncology, The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China | |
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| Abstract
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| [Objective] To observe the impact of prevention effect in different stages of the Yigan Jiangzhi prescription(YGJZP) on D-lactic acid (D-LA) and diamine oxidase (DAO) of alcoholic fatty liver disease (AFLD) of SD rats and analyze the protective mechanism of YGJZP on the intestinal mucosal barrier. [Methods] The 97 SD rats were randomly divided into 22 rats in model group, 75 rats in YGJZF group. According to the different intervention time, YGJZF groups were divided into 3 groups, including the third week medication, the sixth week medication, the ninth week medication. Model group was given fat diet combined with alcohol feeding to produce the model of alcoholic fatty liver. Medicine group was given medicine intervention on the basis of the model group. Finally, rats were respectively sacrificed at different stages of the experiment, to observe the body weight, liver wet weight, liver index of rats, the contents of serum D-LA, DAO, and detect of the contents of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) as well as triglyceride cholesterol (T-CHO) simultaneously. HE staining was used to observe the changes of liver histopathology and the morphology of the intestinal tissue. [Results] With the modeling time prolongs, compared with model group, the body weight and liver wet weights of rats were increased significantly, and the body weight of YGJZF group increased significantly. Compared with the model group, the serum levels of D-LA and DAO in YGJZF group of different periods were significantly lower (P<0.05). The levels of ALT, AST, TG, TC in model group were significantly increased, compared with YGJZF group of different periods. The difference was statistically significant (P<0.05). The activity of D-LA and DAO were correlated with the pathological changes of intestinal tissue were positively correlated trends, and also related with liver histopathological damage. [Conclusion] 1) Preventive administration of YGJZP can protect against the intestinal mucosa, and its possible mechanism may associated with decreasing the serum levels of D-LA, DAO in rats with alcoholic fatty liver. 2) YGJZP can reduce contents of D-LA, DAO and decrease the alcoholic fatty liver damage. 3) YGJZF has played a role in the prevention and protection of intestinal mucosal barrier at different stages among the experimental intervention, especially at the early intervention is more effective. |
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