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| Study on quality standard for Xiongyin Zhongfeng granules |
| Hits 1913 Download times 1616 Received:April 19, 2013 |
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| DOI
10.11656/j.issn.1672-1519.2013.12.15 |
| Key Words
Xiongyin Zhongfeng granules;TLC;Ferulic acid;Paeoniflorin;content determination |
| Author Name | Affiliation | | WANG Chun-sheng | Tianjin Binhai New District Hangu Chinese Medicine Hospital, Tianjin 300480, China | | ZHANG Ling | Tianjin Binhai New District Hangu Chinese Medicine Hospital, Tianjin 300480, China |
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| Abstract
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| [Objective] To establish the quality standard for Xiongyin Zhongfeng granules. [Methods] A identification of Xiongyin Zhongfeng Granules was performed by TLC. The ferulic acid and paeoniflorin were determinated by HPLC. Ferulic acid: It was separated on C18(250 mm×4.6 mm,5μm)column. The mobile phase was methanol-1% acetic acid solution(35:65). The flow rate was 1mL/min. The detection wavelength was 321nm. Paeoniflorin: It was separated on C18(250 mm×4.6 mm,5 μm)column. The mobile phase was acetonitrile-0.01% phosphate solution(15:85). The flow rate was 1 mL/min. The detection wavelength was 230 nm. [Results] Identification of ligusticum chuanxiong hort, angelica, pheretima,paeonia of Xiongyin Zhongfeng granules was established by TLC. The linear range of ferulic acid was 4.286～42.86 μg/mL(r=1.000).The average recovery was 99.7% with RSD=2.8%(n=6). The linear range of paeoniflorin was 0.058 72～0.587 2 mg/mL(r=0.999 99).The average recovery was 99.5% with RSD=0.67%(n=6).[Conclusion] These methods are simple, accurate and repeatable. They can be used as quality standard for Xiongyin Zhongfeng granules. |
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