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| Effects of pachymaran on hematogeneous lung metastasis in mice with melanoma B16 |
| Hits 1934 Download times 1746 Received:November 14, 2013 |
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| DOI
10.11656/j.issn.1672-1519.2014.02.10 |
| Key Words
pachymaran;B16 melanoma mice;lung metastasis;leukocyte |
| Author Name | Affiliation | E-mail | | ZHANG Mi-xia | Tianjin University of TCM, Tianjin 300193, China | | | ZHANG De-sheng | Tianjin University of TCM, Tianjin 300193, China | | | ZHUANG Peng-wei | Tianjin University of TCM, Tianjin 300193, China | | | ZHANG Yan-jun | Tianjin University of TCM, Tianjin 300193, China | zyjsunye@163.com |
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| Abstract
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| [Objective] To investigate the effects and mechanism of pachymaran on lung metastasis in mice with melanoma B16. [Methods] C57BL/6 mice were injected with melanoma B16 cells through tail vein. After establishment of the model, mice were divided into 4 groups with ten mice each. The drug was given through the tail vein every two days in pachymaran high-dose group (pachymaran, 0.5 mg every mouse), pachymaran low-dose group (pachymaran, 0.33 mg every mouse), cisplatin group (cisplatin, 0.04 mg every mouse), and control group (normal saline). The number of metastasis on the surface of the lung was calculated 21 days later. The number of micrometastases in lung was determined by HE staining. We also detected the count of peripheral blood leukocytes and spleen weight, spleen index at the 21st day.[Results] Cisplatin could suppress lung metastases, while reduce the body weight of mice, peripheral blood leukocyte counts, and spleen weight and spleen index. Pachymaran low-dose and high-dose groups had no effect on the body weight of B16 tumor-bearing mice. Pachymaran high-dose group could reduce the number of lung surface metastases. Pachymaran low-dose and high-dose groups could reduce the number of lung micrometastases. Pachymaran high-dose group could significantly decrease the number of the peripheral blood leukocyte but pachymaran low-dose group had no significant effect to it. Pachymaran low-dose group can increase the spleen weight and spleen index of B16 tumor-bearing mice, but low-dose group had no significant effect on them. The spleen weight and spleen index in the Pachymaran low-dose and high-dose groups were higher than the cisplatin group. [Conclusion] Pachymaran can inhibit lung metastases of B16 tumor bearing mice, increase the spleen weight and the spleen index. At the same time, it does not increase the number of the peripheral blood leukocyte. So we speculated that the activation of peripheral blood leukocytes and enhancement of immune function may be involved in the mechanism of the suppression of tumor metastasis of pachymaran. |
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