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| Determination of berberine and palmatine in Jinhuang cream by HPLC |
| Hits 1784 Download times 1407 Received:September 20, 2013 |
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| DOI
10.11656/j.issn.1672-1519.2014.02.12 |
| Key Words
Jinhuang cream;berberine hydrochloride;palmatine;HPLC |
| Author Name | Affiliation | E-mail | | LIU Jun-hong | Tianjin Institute of Acute Abdomen by Integrated Traditional Chinese and Western Medicine, Tianjin 300100, China | | | WANG Hong | The Second Affiliated Hospital of Tianjin University of TCM, Tianjin 300150, China | ctwanghong@sina.com | | LI Di-hua | Tianjin Institute of Acute Abdomen by Integrated Traditional Chinese and Western Medicine, Tianjin 300100, China | | | QU Peng-fei | The Second Affiliated Hospital of Tianjin University of TCM, Tianjin 300150, China | | | LV Yuan-shan | Tianjin Institute of Acute Abdomen by Integrated Traditional Chinese and Western Medicine, Tianjin 300100, China | |
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| Abstract
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| [Objective] To establish a method for simultaneous determination of berberine and palmatine in Jinhuang cream. [Methods] A HPLC method was performed on Apollo C18 (250 mm×4.6 mm, 5 μ) column. The mobile phase consisted of acetonitrile-0.1% phosphoric acid(gradient elution)with the detection wavelengh at 346 nm. The flow rate was 1.0 mL/min and the column temperature was at 30 ℃. [Results] The content of berberine hydrochloride in the range of 0.007 25~0.145 μg and the content of palmatine in the range of 0.005 5~0.110 μg had good linear relationships with the area of eluted peak,and the recovery rate(the average recoveries) was 98.8% and 100.9% respectively. [Conclusion] This method is simple and repeatable, and is suitable for the content determination of berberine and palmatine in Jinhuang cream. |
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