|
| Effects of Astragalus mongholicus on expression of pseudomonas aeruginosa virulence factors and somatic motility |
| Hits 2279 Download times 2124 Received:August 10, 2013 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2014.02.13 |
| Key Words
elastase;pyocyanin;biofilm;somatic motility;Astragalus mongholicus |
| Author Name | Affiliation | E-mail | | YE Li-hua | Department of Infectious Diseases, Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | | | XIA Fei | Wuhan Institute of Biological Institute Co., Ltd., Wuhan 430060, China | | | LI Xin | Department of Gastroenterology, Hanxi Branch of Wuhan First Hospital, Wuhan 430034, China | | | JIANG Bi-wu | Department of Gastroenterology, Hanxi Branch of Wuhan First Hospital, Wuhan 430034, China | | | WANG Ping | Department of Gastroenterology, Hanxi Branch of Wuhan First Hospital, Wuhan 430034, China | 13986271869@139.com |
|
| Abstract
|
| [Objective] To investigate the effects of Astragalus mongholicus on virulence factor of pseudomonas aeruginosa (PA) virulence and somatic motility and explore its mechanism. [Methods] The elastase activity was assessed quantitatively by elastin-Congo red degradation; the secretion of pyocyanin was determined with ultraviolet spectrophotometry after co-culture of different concentration of Astragalus mongholicus and PA at heterogonous grow phase; the biofilm formation was detected by crystal violet staining. The assay of somatic swarming was conducted in petri dishes, containing Astragalus mongholicus. [Results] The expression of elastase and pyocyanin were obviously inhibited at the concentration of 25 g/L, 12.5 g/L and 6.25 g/L of Astragalus mongholicus and the biofilm formation was also inhibited. No effect on bacterial hyperplasia was found under 12.5 g/L. But the swimming and swarming motility of bacteria were reduced at 12.5 g/L. [Conclusion] Astragalus mongholicus can inhibit the hyperplasia and virulence factor of PA at different levels, indicating that the inhibition is controlled by multiple mechanisms, and the quorum sensing system can regulate these factors at a lower concentration of cultivation. |
|
|
|
|
|
|
|
