|
| Simultaneous determination of nine components in Lonicerae Japonicae Flos and Jinqi Jiangtang tablet by HPLC |
| Hits 1958 Download times 2413 Received:September 24, 2013 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2014.03.14 |
| Key Words
Jinqi Jiangtang tablet;Lonicerae Japonicae Flos;HPLC;neochlorogenic acid;cryptochlorogenic acid;isochlorogenic acid A |
| Author Name | Affiliation | E-mail | | CHEN Fang | Tianjin Key Laboratory of Chinese Medicine Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | DENG Yan-ru | Tianjin Key Laboratory of Chinese Medicine Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | dyanru@sina.com | | GUO Li-ping | Tianjin Key Laboratory of Chinese Medicine Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | |
|
| Abstract
|
| [Objective] To develop a HPLC method for simultaneous determination of neochlorogenic acid, cryptochlorogenic acid, chlorogenic acid, caffeic acid, cynaroside, rutin, hyperoside, isochlorogenic acid A and isochlorogenic acid C in Lonicerae Japonicae Flos and Jinqi Jiangtang tablet. [Methods] The chromatographic conditions were follows: Waters Symmetry Shield TM RP18 column (250 mm×4.6 mm, 5 μm), acetonitrile (A) and water ( 0.1% phosphoric acid, B) as mobile phases for gradient elution, 0～5 min,10%～13% A, 5～20 min, 13%～15% A;20～30 min,15%～18% A;30～45 min,18%～20% A;45～52 min,20%～23% A;52～70 min,23% A; the flow rate being 1.0 mL/min. The detection wavelength was set at 327 nm and the column temperature was 25℃。 [Results] The results showed that nine components were well separated and showed good linearity. The average recoveries were between 95%～105%. [Conclusion] This is a specific, sensitive, repeatable and simple method for simultaneous determining neochlorogenic acid, cryptochlorogenic acid, chlorogenic acid, caffeic acid, cynaroside, rutin, hyperoside, isochlorogenic acid A and isochlorogenic acid C in Lonicerae Japonicae Flos and Jinqi Jiangtang tablet. The method can be available for the quality control of Jinqi Jiangtang tablet. |
|
|
|
|
|
|
|
