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| HPLC method for determining ginsenoside content in Kangshen oral liquid |
| Hits 1640 Download times 1287 Received:October 12, 2013 |
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| DOI
10.11656/j.issn.1672-1519.2014.03.17 |
| Key Words
HPLC;Kangshen oral liquid;ginsenoside;content determination |
| Author Name | Affiliation | E-mail | | HAO Shao-jun | The 371st Hospital Center of Chinese People's Liberation Army, Xinxiang 453000, China | | | LI Wen-jun | The 371st Hospital Center of Chinese People's Liberation Army, Xinxiang 453000, China | | | FAN Li-li | The 371st Hospital Center of Chinese People's Liberation Army, Xinxiang 453000, China | | | ZHANG Zheng-chen | The 371st Hospital Center of Chinese People's Liberation Army, Xinxiang 453000, China | xxzzc2010@126.com |
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| Abstract
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| [Objective] To study the content of the ginsenoside in Kangshen oral liquid by HPLC method. [Methods] Take eighteen alkyl silane bonded silica as filler, water solution with acetonitrile (82:18) as mobile phase; the detection wavelength was 203 nm. Number of theoretical plates by ginsenoside Rg1 peak calculation should not be less than 6000. [Results] The ginsenoside Rg1 concentration in the range of 0.053~0.265 g/L, ginseng saponin Re concentration in the range of 0.037~0.185 g/L, the peak area and the concentration had good linear relationship. The average recovery rate was 99.12%. [Conclusion] The HPLC method for the determination of ginsenoside content in Kangshen oral liquid has a feature of simple operation, good reproducibility. It can effectively control the quality standard of Kangshen oral liquid. |
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