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| Simultaneous determination of free anthraquinones in Rhubarb fromdifferent habitats by UPLC |
| Hits 1911 Download times 1347 Received:December 13, 2013 |
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| DOI
10.11656/j.issn.1672-1519.2014.04.15 |
| Key Words
Rhubarb;determination;UPLC |
| Author Name | Affiliation | E-mail | | LIU Bing-wang | Department of Molecular Imaging and Nuclear Medicine, Tianjin Medical University Cancer Institute and Hospital, National ClinicalResearch Center of Cancer, Key Laboratory of "Cancer Prevention and Therapy", Tianjin 300060, China | | | XU Wen-gui | Department of Molecular Imaging and Nuclear Medicine, Tianjin Medical University Cancer Institute and Hospital, National ClinicalResearch Center of Cancer, Key Laboratory of "Cancer Prevention and Therapy", Tianjin 300060, China | wenguixy@tom.com | | Wang Jian | Department of Molecular Imaging and Nuclear Medicine, Tianjin Medical University Cancer Institute and Hospital, National ClinicalResearch Center of Cancer, Key Laboratory of "Cancer Prevention and Therapy", Tianjin 300060, China | |
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| Abstract
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| [Objective] To develop a UPLC method for simultaneous determination of five anthraquinones (Rhein,Chrysophanol,Emodin,Physcion and Aloe-emodin) in Radix et Rhizom a Rhei from different habitats. [Methods] The analysis was performed on Waters BEH C18 column (2.1 mm×150 mm,1.8 μm) with a gradient mobile phase of acetonitrile (A)-0.1% Formic acid solution (B) (0～3 min,19%→25%A,3～10 min,25%→100%A) at flow rate of 0.3 mL/min. The column was maintain at 30℃ and the detection wavelength was set at 254 nm. [Results] The linear ranges were 1.61～80.30 μg/mL for Rhein,4.98～249.20 μg/mL for Chrysophanol,3.27～163.30 μg/mL for Emodin,9.65～482.50 μg/mL for Physcion and 1.67～83.30 μg/mL for Aloe-emodin. The average recoveries of the five compounds were 99.7%,100.4%,100.1%,98.9%,100.9% and RSD were 1.5%,0.6%,0.8%,0.4%,1.7% respectively.[Conclusion] The rapid and accurate UPLC method was established. This method can be used to assess the quality of Rhubarb. |
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