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| Study on acid hydrolytic conditions of total flavonoids and the determination of flavonoid aglycones from Exochorda serratifolia S. Moore. Leaves |
| Hits 1661 Download times 1065 Received:December 25, 2013 |
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| DOI
10.11656/j.issn.1672-1519.2014.05.18 |
| Key Words
Exochorda serratifolia S. Moore;flavonoids;acid hydrolysis;content determination |
| Author Name | Affiliation | | LI Gu-yue | The First Affiliated Hospital of China Medical University, Shenyang 110000, China
Pharmacy College of China Medical University, Shenyang 110000, China | | LIU Jia-xin | The First Affiliated Hospital of China Medical University, Shenyang 110000, China
Pharmacy College of China Medical University, Shenyang 110000, China | | CAI Qian | Pharmacy College of Liaoning University of Traditional Chinese Medicine, Dalian 116600, China |
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| Abstract
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| [Objective] To establish the acid hydrolytic conditions of total flavonoids and to determine the flavonoid aglycones from Exochorda serratifolia S. Moore. leaves. [Methods] The acid hydrolytic conditions were optimized by the means of orthogonal test and the contents of the flavonoid aglycones were determined by HPLC. [Results] The optimum conditions of acid hydrolysis were: concentration of hydrochloric acid 4.0 mol/L, temperature 78 ℃， time 4 h. The contents of luteolin, quercetin and apigenin were 12.06%±0.32%, 9.66%±1.43% and 36.66%±2.34% respectively. [Conclusion] This method is accurate and reliable and can be used in the determination of the flavonoid aglycones in Exochorda serratifolia S. Moore. leaves. |
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