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| Effects of Transcutol P on pharmacokinetics of Tetrahydropulmatine in Tongning gel in rats |
| Hits 1928 Download times 1507 Received:January 05, 2014 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2014.06.14 |
| Key Words
Tetrahydropulmatine|Tongning gel|UPLC-MS/MS|pharmacokinetics |
| Author Name | Affiliation | E-mail | | PANG Xiao-chen | Tianjin University of Traditional Chinese Medicine, Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin 300193, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China | | | LIU Zhi-dong | Tianjin University of Traditional Chinese Medicine, Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin 300193, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China | lonerliuzd@163.com | | GUO Xiu-jun | Tianjin University of Traditional Chinese Medicine, Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin 300193, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China | | | GUO Li-li | Tianjin University of Traditional Chinese Medicine, Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin 300193, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China | | | YIN Zhong-peng | Tianjin University of Traditional Chinese Medicine, Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin 300193, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China | | | ZHANG Qian | Tianjin University of Traditional Chinese Medicine, Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin 300193, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China | | | Xiao Wei | Jiangsu Kanion Pharmaceut Co. Ltd. , Lianyungang 222001, China
State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China | |
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| Abstract
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| [Objective] To establish a highly sensitive UPLC-MS/MS method for the quantification of plasma Tetrahydropalmatine in rats, investigate the effects of 7%Transcutol P on the pharmacokinetics of Tetrahydropulmatine in Tongning gel in rats and study its promoting effect for transdermal absorption. [Methods] Using a method of UPLC-MS/MS to measure plasma concentration of Tetrahydropulmatine at different time after transdermal administration a pharmacokinetics comparison with the results of control group was performed. [Results] The pharmacokinetic parametersTmax and Cmax between these two groups of 7%Transcutol Pand control were statistically significant (p<0.05). The AUC(0→24 h) and UC(0→∞) values were higher than those of the control solution, respectively with statistically significant difference (P<0.05). [Conclusion] This UPLC-MS/MS method is successfully applied in the determining of plasma Tetrahydropalmatine concentration in rats. The pharmacokinetic parameters have indicated that the enhancing effect of 7% Transcutol P is significant and can be further used in the preparation of Tongning gel. |
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