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| Effects of electroacupuncture on expression of VEGF/Flt-1 mRNA and protein in rats with acute cerebral infarction |
| Hits 1926 Download times 1598 Received:April 20, 2014 |
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| DOI
10.11656/j.issn.1672-1519.2014.07.13 |
| Key Words
cerebral infarction;VEGF;Flt-1;angiogenesis;Renzhong point |
| Author Name | Affiliation | E-mail | | LI Jing | The Acupuncture and Moxibustion Institute of the First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | MA Ying | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | HE Jiao-jun | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | CUI Jing-jun | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | DU Yuan-hao | The Acupuncture and Moxibustion Department of the First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | jpjs_cn@sina.com. |
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| Abstract
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| [Objective] To identify the intervention mechanism of the acupuncture on cerebral angiogenesis by investigating the effects of electroacupuncture on VEGF/Flt-1 of rats with acute cerebral infarction. [Methods] The acute cerebral infarction in rat was reproduced by MCAO (Middle cerebral artery occlusion). The 120 Wister rats were divided into 3 groups: control group, model group and electroacupuncture group. Two groups were performed according to the continued time after the instant of MCAO and were divided into 3 h phase G, 6 h phase G, 12 h phase G, 24 h phase G, 3 d phase G, 7 d phase G, 12 d phase G, having 8 rats in each phase group. The electroacupuncture group was electroacupunctured in the Renzhong(DU26) point instantly after operation. The rats were killed at the time according to the phase, and detected the mRNA and protein expression of the VEGF/flt-1with the method of Real-Time PCR (Taqman) and immunohistochemestry. [Results] The expression of VEGF mRNA and protein in the electroacupuncture group was significant higher than that in the model group. The expression tendency of Flt-1mRNA in the electroacupuncture philtrum group was smooth compared with the model group. Only the expression of Flt-1 protein in the electroacupuncture group was positive. [Conclusion] The results suggest that the electroacupuncture can optimum regulate the expression of mRNA and protein of VEGF/flt-1 and improve the angiogenesis of ischemic penumbra. |
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