|
| Effect of Xifeng capsule on hippocampal synaptic injury in epileptic rat induced by lithium chloride-pilocarpine |
| Hits 1956 Download times 1334 Received:February 02, 2014 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2014.07.14 |
| Key Words
epilepsy;Xifeng capsule;synaptophysin;growth associated protein-43 |
| Author Name | Affiliation | E-mail | | LU Yan-li | Pediatrics Department, The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Zhen | Children's Hospital of Baoding, Baoding 071000, China | | | MA Li-ting | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Xin-min | Pediatrics Department, The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | antiepilepsia@sohu.com |
|
| Abstract
|
| [Objective] To observe the intervention effect of Xifeng capsule only and combined therapy on hippocampal synaptic injury in epileptic rats induced with lithium chloride- pilocarpine. [Methods] The 64 SPF healthy male Wistar rats were randomly divided into normal group, model group, high dose of Xifeng capsule group (high group), medium dose of Xifeng capsule group (medium group), low dose of Xifeng capsule group (low group), high dose of Xifeng capsule+topiramate group (combined high group), high dose of Xifeng capsule + 1/2 dose of topiramate group (combined low group), topiramate group (TPM group). Every group had 8 rats. Applying the ignited method with lithium chloride-pilocarpine chemicals a model of epilepsy was reproduced in rats. Using SABC immunohistochemistry the synapse cable (P38) expression levels was detected to reflect the growth of synapses cable. The expression level of growth of associated protein-43 (GAP-43) mRNA was detected bu in situ hybridization to reflect the level of growth associated protein-43. [Results] 1)The experimental results showed that some visible point patchy distributed as stained granular immune reaction products was found in hippocampal CA1, CA3 and dentate gyrus molecular layer and the granule cell layer in all epileptic rats. The total area of P38 immune reaction products in hippocampal CA1, CA3 and dentate gyrus starting layer molecular layer of all treatment groups was significantly lower than that of the model group (P<0.01). The total area of P38 immune reaction products in hippocampal CA1, CA3 and dentate gyrus starting layer molecular layer of combined high group had no significant difference when compared with that of the normal group (P>0.05). Positive reaction products in hippocampal CA1 and CA3 areas of high group had no significant difference compared with the normal group also (P>0.05). In the hippocampal CA1 region combined high group and high group it was better than the low group and the rest of the treatment groups (P<0.05). In the hippocampal CA3 combined high group it was superior to the low group and TPM group (P<0.05), but showed no significant difference when compared with the rest of the treatment groups (P>0.05). In the dentate gyrus region, combined high group was significantly different compared with the rest of the treatment groups (P<0.05). 2)Hippocampal granule cells GAP-43 mRNA of all treatment groups was significantly lower than that of the model group (P<0.01). Combined high group had no significant difference with high group in hippocampal CA1 region (P>0.05), but was better than medium group and low group (P<0.05). In the CA3 region, there were no significant differences among all treatment groups (P>0.05). In the dentate gyrus region, combined high group and high group were significantly different compared with medium group, low group and TPM group (P<0.05). [Conclusion] Xifeng capsule only and combined therapy can effectively intervene the expression of growth-associated protein-43 in hippocampal granule cell layer and inner molecular layer synapses It plays a role in rats with epilepsy induced by lithium chloride-pilocarpine for reducing the hippocampal damage. |
|
|
|
|
|
|
|
