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| Neuroprotective role and its mechanism of Shenfu injection on focal ischemic stroke in rats |
| Hits 1787 Download times 1780 Received:February 21, 2014 |
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| DOI
10.11656/j.issn.1672-1519.2014.07.15 |
| Key Words
Shenfu injection;ischemic stroke;Na-K-Cl-cotransporter 1 |
| Author Name | Affiliation | E-mail | | YANG Yong | Prescription Department, Beijing University of Chinese Medicine, Beijing 100029, China | | | REN Chang-hong | Hypoxia Medical Research Laboratory, Xuanwu Hospital of Capital Medical University, Beijing 100053, China | | | WU Xiao-dan | Prescription Department, Beijing University of Chinese Medicine, Beijing 100029, China | wutun318@163.com. |
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| Abstract
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| [Objective] To investigate the effect of Shenfu injection on the expression of Na-K-Cl-cotransporter 1 (NKCC1) in rats with focal cerebral ischemic stroke. [Methods] The ischemic stroke was generated by 90 min occlusion of right middle cerebral artery. After 24 h reperfusion, neurological score was evaluated. The cerebral infarct volumes were quantified by 2, 3, 4-triphenytetrazolium-chloride (TTC), and the NKCC1expression were evaluated by Western blot and immunofluorescence methods. [Results] Shenfu injection-treatment group (SFI) significantly reduced the infarct volume (P<0.05) and the degree of neurological deficits (P<0.05). Compared to the ischemia group, the protein expression of NKCC1 was significantly reduced in the SFI group. [Conclusion] Shenfu injection plays a neuroprotective role in rats with experimental cerebral ischemia, which was associated with the inhibition of NKCC1. |
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