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| Simultaneous determination of seven components in medicinal and non-medicinal parts of Forsythia suspensa by RP-HPLC |
| Hits 1898 Download times 1867 Received:February 11, 2014 |
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| DOI
10.11656/j.issn.1672-1519.2014.07.16 |
| Key Words
HPLC;Forsythia suspensa (Thunb.) Vahl;forsythoside A;forsythoside B;astragalin;forsythin |
| Author Name | Affiliation | E-mail | | LI Jin | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Chun-peng | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | MA Lin | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZHANG Jian | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | HU Jing | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | CHANG Yan-xu | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | tcmcyx@126.com |
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| Abstract
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| [Objective] To establish an HPLC method for the simultaneous determination of forsythoside A, forsythoside B, astragalin, forsythin, arctiin, quercetin and arctigenin in Medicinal and non-medicinal parts of Forsythia suspensa. [Methods] The analysis was performed on an Agilent Eclipse Plus C18(4.6×100 mm, 1.8 μm). The mobile phase was composed of acetonitrile and 0.1% formic acid with gradient elution. The detection wavelength was set at 280 nm. The flow rate was 0.3 mL/min at column temperature of 30℃. [Results] Forsythoside A, forsythoside B, astragalin, forsythin, arctiin, quercetin and arctigenin were well separated by HPLC and showed a good linearity ranges. The average recoveries of the seven components were 100%, 99.1%, 104%, 104%, 98.4%, 99.0% and 103% and the RSD values were 2.27%, 1.59%, 1.94%, 1.25%, 2.97%, 2.10% and 2.67%, respectively. [Conclusion] A rapid and simple HPLC method was established to simultaneously determine the contents of the seven components in Forsythia suspense, which provides a scientific basis for the quality control of Forsythia suspense. |
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