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| Change of hepatic energy metabolismin rats with deficiency cold status |
| Hits 1723 Download times 1277 Received:March 21, 2014 |
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| DOI
10.11656/j.issn.1672-1519.2014.08.13 |
| Key Words
deficiency cold status;energy metabolism;hydrocortisone |
| Author Name | Affiliation | E-mail | | LI Lian-zhen | Agronomy College, Henan Agricultural University, Zhengzhou 450002, China | | | XUE Chun-miao | Dongzhimen Hospital Affiliated to Beijing University of Traditional Chinese Medicine, Beijing 100700, China | | | ZHANG Bing | School of Chinese Material Medica, Beijing University of Traditional Chinese Medicine, Beijing 100029, China | zhangbing6@263.net | | LIU Xiao-qing | School of Chinese Material Medica, Beijing University of Traditional Chinese Medicine, Beijing 100029, China | |
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| Abstract
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| [Objective] To investigate the change of hepatic energy metabolism in rats with deficiency cold induced by hydrocortisone. [Methods] Forty eight male SD rats were divided into two groups. The experimental group was intramuscularly injected with hydrocoriti-sone sodium succinate saline, 20 mg/kg and the control group was intramuscularly injected with saline, 0.25 mL. Five mL blood sample was collected from abdominal main vein on 15th day and 22th day. Serum LAC level were detected by automatic biochemical analyzer. The activities of Na+-K+-ATPase, Mg2+-ATPase, Ca2+-ATPase, Ca2+-Mg2+-ATPase and SDH were detected by ultraviolet spectrophotometry. The protein expression of hepatic uncoupling protein 2 (UCP2) was detected by immunohistochemistry. [Results] Compared with those in control group, the activities of serum LAC and hepatic tissue SDH were significantly lower P<0.05 on 14th day and 21th day, the activi-ties of hepatic tissue Na+-K+-ATPase, Mg2+-ATPase, Ca2+-ATPase, Ca2+-Mg2+-ATPase, the positive expression areas and integral optical density of UCP-2 were significantly lower P<0.05, P<0.01 on 21th day. The other indexes had no obvious change. [Conclusion] During the experimental process, the function of hepatic energy metabolism is significantly lower or disordered in animals with deficiency cold induced by hydrocortisone. |
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