|
| Effect of coumarins extract of Angelica dahurica, tetramethylpyrazine and compatible application on cerebral isxhemia/reperfusion injury in rats |
| Hits 2583 Download times 1413 Received:April 02, 2014 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2014.09.15 |
| Key Words
total coumarins extract of Angelica dahurica;tetramethylpyrazine;cerebral ischemia/reperfusion;lipid peroxidation;superoxide dismutase;malondialdehyde |
| Author Name | Affiliation | E-mail | | ZHONG Pei-ru | College of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZHANG Fang-liang | College of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | HE Li-na | College of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LIU Sha-sha | College of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | Fan Yao-wen | College of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | HE Xin | College of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | hexintn@163.com |
|
| Abstract
|
| [Objective] To investigate the individual and compatible effects of coumarins extracts of Angelica dahurica and tetram-ethylpyrazine(TMP) on cerebral ischemia/reperfusion(I/R) injury in rats. [Methods] Male healthy SD rats were randomly divided into six groups: sham group, I/R group, TMP group (7.5 mg/kg tetramethylpyrazine), TCE group (50 mg/kg total coumarins extract of Angelica dahurica), TMP-TCE group (7.5 mg/kg+50 mg/kg) and Nimodipine group (10 mg/kg). Rats were pretreated with test drugs for sequential seven days by intragastric administration and underwent focal cerebral ishchemia for 2 h and reperfusion for 22 h. The neurofunctional scores were evaluated and the infarct volume was measured by triphenyltetrazolium chloride (TTC) staining. The activity of superoxide dismutase (SOD) and malondialdehyde (MDA) content in brain were determined to investigate the mechanism. [Results] Compared with I/R group, the neurological deficit in TMP, TCE and TMP-TCE group was significantly improved (P<0.05), meanwhile the cerebral infarct volume of TMP-TCE group was reduced significantly (P<0.05). The activity of SOD was enhanced (P<0.05 or P<0.01), and the content of MDA was decreased (P<0.01). [Conclusion] Individual and compatible application of the total coumarins extract of Angelica dahurica and tetramethylpyrazine have a protective effect against the ischemic stroke, which might be related with inhibiting the lipid peroxidation of the brain tissue. |
|
|
|
|
|
|
|
